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KMID : 0352819940100010041
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Kosin Medical Journal
1994 Volume.10 No. 1 p.41 ~ p.48
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Effect of serum on biosynthesis of proteoglycan of human articular cartilage in culture.
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Abstract
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Articular cartilage is a highly specialized connective tissue that covers the ends of long bones within the synovial joint cavity. It contains no blood vessels, no nerve fibers, and no lymphatics, accordingly it is nourished from the synovlal
fluid
by
diffusion.
Articular cartilage is composed of an extracellular matrix(ECM). collagen and proteoglycan. A small number of specific cells, chondrocytes, are embedded in the matrix. They are influenced by the alteration of components in synovial fluid which is
derived from serum.
To examine the effect of serum on biosynthesis of proteoglycan, this experiment was performed using human articular cartilages which were obtained from the patients who were operated due to various dieseases. The human articular cartilages were
incubated in 3 kinds of media : DMEM without fetal calf serum(FCS) (control), DMFM containing 1% FCS, and DMEM containing 20% FCS. The synthethized proteoglycan, incorporated with labeled S35sulfate, was extracted through PD-10 column after
digestion by
papain and measured the uptake of [35S] sulfate labeled proteoglycan by scintillation conuter. To examine whether difference in concentration of fetal calf serum is reflected in staining intensity of immunohistochemistry for proteoglycan
(avidin-biotin
peroxidase complex), immunohistochemical staining was performed.
@ES The results were as followings:
@EN 1) Biosynthese of proteoglycan in 1% and 20% FCS were increased 1.77 times and 22.7 respectively compairing with the control group.
2) In FCS groups, biosynthesis of proteoglycan in 205 FCS was 1.29 times higher than that in 1% FCS.
3) No difference-in staining intensity for proteoglycan in the variable concentrations of fetal calf serum was seen.
The above results that the compositional alteration of synovial fluid due to various factors influence the synthesis of the ECM in the articular cartilage and immunohistochemical staining is not sensitive enough to differentiate the quantitative
difference of antigen.
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KEYWORD
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